In studies of prostate cancer, researchers have used cell lines generated from patients with the most virulent forms of cancer - the kind that has metastasized to invade bone or lymph nodes. But these do not have the same characteristics as cells that come from a localized prostate tumor. The goal of our laboratory work is to develop a cancer cell line that will have the characteristics of the prostate tumor "in vivo." Having this new source of a prostate cancer cell line will benefit prostate cancer research, including the development of drugs. To accomplish this goal we are generating prostate cancer cell lines from tumor tissue of a prostatectomy specimen. The genotypes will be analyzed, and the cell lines taken from the "in vivo" tumor will be used in future research.